The stem cell is now a new subject to research with a high future prospect. Researchers claim that it has an immense power within itself to cure many complicated diseases including various types of cancer. Stem cell preservation is still not a very common thing to people but eventually, it is getting the popularity. The researchers about it are still not over and there are many more things to know about it. Stem cells are present in the bone marrow of the human body but it is not always possible to collect the stem cells from the bone marrow. On the other hand, collecting stem cells from the umbilical cord during delivery is a much easier process and is not painful at all.
The process of preserving the umbilical cord stem cells is somehow a complicated process. Collecting the stem cells is not the end of the process. The cells must stay viable in order to get used. The stem cell therapy in india is now becoming a vast field for research and new inventions. That is why there are not many varieties in the storage and collection procedure. The steps performed in collecting and preserving the stem cell is as follows:
Step 1: At first, a part of the baby’s umbilical cord is cut and the lining of the cord is collected. This is just an extension part of collecting the cord blood of the baby. After the collection of the blood, a portion from the umbilical cord is cut and stored into a sterile vessel. The vessel is provided by the collection kit and sterilized by various laboratory processes.
Step 2: The further procedure is done in the laboratory under the supervision of the lab attendant. It should be done under the extreme sterilized condition to lower the chances of any contamination. Before doing the further processes, the laboratory technician checks the identity of the blood and make sure that the right blood is going under the name of the right client. After this thing is done the sample is cryopreserved.
Step 3: In the next process the cord lining is washed with a buffer solution to remove any contaminant and then cut into smaller segments. These segments are then kept in vials and a protectant solution is added to it to maintain the viability during the process.
Step 4: Then comes the most important step, cooling. In this process, the segments are frozen in a controlled manner. The temperature is lowered gradually in a freezer. Generally, 1-2 degree is lowered per minute. This helps in keeping the viability of the cells. If the temperature is lowered in one shot then cells would have died.
Step 5: After the cells are cooled down to a certain temperature in a controlled manner the vials are then kept in a storage system. Cryopreservation is done for a long time and that is why it is kept in the liquid nitrogen storage which is in the vapour phase. The temperature of the liquid nitrogen storage system is -197 degree centigrade.